recombinant human slpi protein Search Results


recombinant human slpi  (R&D Systems)
94
R&D Systems recombinant human slpi
Expression levels of the nanobodies targeting HE4, <t> SLPI, </t> and PGRN and equilibrium dissociation constants (K D ), as measured by indirect ELISA and expressed by the EC50 value. Clones expressed at levels >25 mg/L and showing a K D < 50 nM were further studied using SPR to assess their binding affinity.
Recombinant Human Slpi, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human slpi/product/R&D Systems
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recombinant human slpi - by Bioz Stars, 2026-02
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Expression levels of the nanobodies targeting HE4, SLPI, and PGRN and equilibrium dissociation constants (K D ), as measured by indirect ELISA and expressed by the EC50 value. Clones expressed at levels >25 mg/L and showing a K D < 50 nM were further studied using SPR to assess their binding affinity.

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Expression levels of the nanobodies targeting HE4, SLPI, and PGRN and equilibrium dissociation constants (K D ), as measured by indirect ELISA and expressed by the EC50 value. Clones expressed at levels >25 mg/L and showing a K D < 50 nM were further studied using SPR to assess their binding affinity.

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques: Expressing, Indirect ELISA, Clone Assay, Binding Assay

Indirect ELISA results showing the antigen-binding capacities of the nanobody variants targeting HE4 ( top ), SLPI ( middle ), and PGRN ( bottom ).

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Indirect ELISA results showing the antigen-binding capacities of the nanobody variants targeting HE4 ( top ), SLPI ( middle ), and PGRN ( bottom ).

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques: Indirect ELISA, Binding Assay

Antigen-binding affinity of Nbs HE4-1 ( top ), SLPI-7 ( middle ), and PGRN-7 ( bottom ), as determined by SPR (k on in M −1 .s −1 and k off in s −1 ).

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Antigen-binding affinity of Nbs HE4-1 ( top ), SLPI-7 ( middle ), and PGRN-7 ( bottom ), as determined by SPR (k on in M −1 .s −1 and k off in s −1 ).

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques: Binding Assay

Non-competitive Nb pair possibilities for SLPI (C—competitive; P.C—partially competitive; N.C—non-competitive).

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Non-competitive Nb pair possibilities for SLPI (C—competitive; P.C—partially competitive; N.C—non-competitive).

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques:

Sandwich ELISA plots showing the detection results of the recombinant human antigens HE4, SLPI, and PGRN by their respective best-performing nanobodies: HE4-6 in PBS ( A ) and serum ( B ); SLPI-1 in PBS ( C ) and serum ( D ); and PGRN-5 in PBS ( E ) and serum ( F ). The yellow line indicates the LOD.

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Sandwich ELISA plots showing the detection results of the recombinant human antigens HE4, SLPI, and PGRN by their respective best-performing nanobodies: HE4-6 in PBS ( A ) and serum ( B ); SLPI-1 in PBS ( C ) and serum ( D ); and PGRN-5 in PBS ( E ) and serum ( F ). The yellow line indicates the LOD.

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques: Sandwich ELISA, Recombinant

Comparison between the serum levels in healthy women and the obtained limits of detection (LOD) for the recombinant human HE4, SLPI, and PGRN antigens by the selected nanobodies HE4-6, SLPI-1, and PGRN-5, respectively.

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Comparison between the serum levels in healthy women and the obtained limits of detection (LOD) for the recombinant human HE4, SLPI, and PGRN antigens by the selected nanobodies HE4-6, SLPI-1, and PGRN-5, respectively.

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques: Comparison, Recombinant

Antigen dilution series for each Nb (dilutions in PBS and human serum).

Journal: International Journal of Molecular Sciences

Article Title: Nanobodies for the Early Detection of Ovarian Cancer

doi: 10.3390/ijms232213687

Figure Lengend Snippet: Antigen dilution series for each Nb (dilutions in PBS and human serum).

Article Snippet: Human WAP5/WDFC2/HE4 protein (His tag) antigen was purchased from Bioconnect (Huissen, The Netherlands), and recombinant human SLPI and PGRN antigens were purchased from R&D systems (Abington, UK).

Techniques: Concentration Assay